Schistosoma mansoni: Genetic Restriction and Cytokine Profile of the CD4 + T Helper Cell Response to Dominant Epitope Peptide of Major Egg Antigen Sm-p40
Identifieur interne : 001336 ( Istex/Checkpoint ); précédent : 001335; suivant : 001337Schistosoma mansoni: Genetic Restriction and Cytokine Profile of the CD4 + T Helper Cell Response to Dominant Epitope Peptide of Major Egg Antigen Sm-p40
Auteurs : Hector J. Hernandez [États-Unis] ; Clark M. Edson [États-Unis] ; Donald A. Harn [États-Unis] ; Christopher J. Ianelli [États-Unis] ; Miguel J. Stadecker [États-Unis]Source :
- Experimental Parasitology [ 0014-4894 ] ; 1998.
English descriptors
- KwdEn :
- Teeft :
- Amino, Amino acids, Antigen, Background values, Boros, Cell epitopes, Cell hybridoma responses, Cell hybridomas, Cell proliferative responses, Cell response, Clonal level, Culture supernatants, Cytokine, Cytokine profile, Dominant epitope peptide, Epitope, European journal, Granuloma, Granuloma formation, Hybridoma, Immunology, Lymph, Lymph node cells, Major schistosomal, Mansoni, Mesenteric lymph nodes, Mouse, Mouse strains, Node, Peptide, Polyclonal, Proliferative, Schistosoma, Schistosoma mansoni, Schistosomal, Similar results, Stadecker, Stimulatory, Stimulatory peptides, Triplicate cultures, Tropical diseases, Various mouse strains.
Abstract
Abstract: Hernandez, H. J., Edson, C. M., Harn, D. A., Ianelli, C. J., and Stadecker, M. J. 1998.Schistosoma mansoni: Genetic restriction and cytokine profile of the CD4+ T helper cell response to dominant epitope peptide of major egg antigen Sm-p40.Experimental Parasitology90, 122–130. Granuloma formation in schistosomiasis is mediated by MHC class II-restricted CD4 + T helper lymphocytes sensitized to egg antigens. We previously reported that C3H mice, which develop large granulomas, display strong CD4 + T helper cell responses to the major egg antigen Sm-p40. Moreover, all members of a panel of egg antigen-specific T cell hybridomas responded to the Sm-p40 antigen. Given the significance of the Sm-p40 molecule in the C3H T cell repertoire against schistosomal egg antigens, the current work was undertaken to map its immunogenic epitopes, using a library of 15 synthetic overlapping 30-mer peptides. The dominant epitope recognized by polyclonal CD4 + Th cells was located in peptide 10 (amino acids 229–258); subdominant epitopes were detected in peptides 8 (amino acids 179–208) and 12 (amino acids 279–308). The anti-Sm-p40 T cell hybridomas variously responded to any one of the same three stimulatory peptides. Furthermore, studies with various mouse strains demonstrated that a strong anti-Sm-p40 response was restricted by H-2k. Interestingly, the cells responding to peptide 10 and to the Sm-p40 antigen only secreted IL-2 and IFN-γ, but not IL-4 and IL-10, indicating that they are entirely of the Th-1-type, a subset with demonstrated capacity to mediate egg granuloma formation. The identification of dominant epitopes within key egg antigens offers opportunities for desensitization of the CD4 + Th cells that mediate pathology in schistosomia sis.
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DOI: 10.1006/expr.1998.4309
Affiliations:
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Ianelli</name></author><author><name sortKey="Stadecker, Miguel J" sort="Stadecker, Miguel J" uniqKey="Stadecker M" first="Miguel J." last="Stadecker">Miguel J. 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Hernandez</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts, 02111</wicri:regionArea><wicri:noRegion>02111</wicri:noRegion></affiliation></author><author><name sortKey="Edson, Clark M" sort="Edson, Clark M" uniqKey="Edson C" first="Clark M." last="Edson">Clark M. Edson</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts, 02111</wicri:regionArea><wicri:noRegion>02111</wicri:noRegion></affiliation></author><author><name sortKey="Harn, Donald A" sort="Harn, Donald A" uniqKey="Harn D" first="Donald A." last="Harn">Donald A. Harn</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts, 02115</wicri:regionArea><wicri:noRegion>02115</wicri:noRegion></affiliation></author><author><name sortKey="Ianelli, Christopher J" sort="Ianelli, Christopher J" uniqKey="Ianelli C" first="Christopher J." last="Ianelli">Christopher J. Ianelli</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts, 02111</wicri:regionArea><wicri:noRegion>02111</wicri:noRegion></affiliation></author><author><name sortKey="Stadecker, Miguel J" sort="Stadecker, Miguel J" uniqKey="Stadecker M" first="Miguel J." last="Stadecker">Miguel J. Stadecker</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts, 02111</wicri:regionArea><wicri:noRegion>02111</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Parasitology</title><title level="j" type="abbrev">YEXPR</title><idno type="ISSN">0014-4894</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">90</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="122">122</biblScope><biblScope unit="page" to="130">130</biblScope></imprint><idno type="ISSN">0014-4894</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4894</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>MHC restriction</term><term>SEA, schistosomal egg antigens.</term><term>Schistosoma mansoni</term><term>T cell epitopes</term><term>T cell subsets</term><term>cytokines</term><term>egg antigens</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acids</term><term>Antigen</term><term>Background values</term><term>Boros</term><term>Cell epitopes</term><term>Cell hybridoma responses</term><term>Cell hybridomas</term><term>Cell proliferative responses</term><term>Cell response</term><term>Clonal level</term><term>Culture supernatants</term><term>Cytokine</term><term>Cytokine profile</term><term>Dominant epitope peptide</term><term>Epitope</term><term>European journal</term><term>Granuloma</term><term>Granuloma formation</term><term>Hybridoma</term><term>Immunology</term><term>Lymph</term><term>Lymph node cells</term><term>Major schistosomal</term><term>Mansoni</term><term>Mesenteric lymph nodes</term><term>Mouse</term><term>Mouse strains</term><term>Node</term><term>Peptide</term><term>Polyclonal</term><term>Proliferative</term><term>Schistosoma</term><term>Schistosoma mansoni</term><term>Schistosomal</term><term>Similar results</term><term>Stadecker</term><term>Stimulatory</term><term>Stimulatory peptides</term><term>Triplicate cultures</term><term>Tropical diseases</term><term>Various mouse strains</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Hernandez, H. J., Edson, C. M., Harn, D. A., Ianelli, C. J., and Stadecker, M. J. 1998.Schistosoma mansoni: Genetic restriction and cytokine profile of the CD4+ T helper cell response to dominant epitope peptide of major egg antigen Sm-p40.Experimental Parasitology90, 122–130. Granuloma formation in schistosomiasis is mediated by MHC class II-restricted CD4 + T helper lymphocytes sensitized to egg antigens. We previously reported that C3H mice, which develop large granulomas, display strong CD4 + T helper cell responses to the major egg antigen Sm-p40. Moreover, all members of a panel of egg antigen-specific T cell hybridomas responded to the Sm-p40 antigen. Given the significance of the Sm-p40 molecule in the C3H T cell repertoire against schistosomal egg antigens, the current work was undertaken to map its immunogenic epitopes, using a library of 15 synthetic overlapping 30-mer peptides. The dominant epitope recognized by polyclonal CD4 + Th cells was located in peptide 10 (amino acids 229–258); subdominant epitopes were detected in peptides 8 (amino acids 179–208) and 12 (amino acids 279–308). The anti-Sm-p40 T cell hybridomas variously responded to any one of the same three stimulatory peptides. Furthermore, studies with various mouse strains demonstrated that a strong anti-Sm-p40 response was restricted by H-2k. Interestingly, the cells responding to peptide 10 and to the Sm-p40 antigen only secreted IL-2 and IFN-γ, but not IL-4 and IL-10, indicating that they are entirely of the Th-1-type, a subset with demonstrated capacity to mediate egg granuloma formation. The identification of dominant epitopes within key egg antigens offers opportunities for desensitization of the CD4 + Th cells that mediate pathology in schistosomia sis.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Hernandez, Hector J" sort="Hernandez, Hector J" uniqKey="Hernandez H" first="Hector J." last="Hernandez">Hector J. Hernandez</name></noRegion><name sortKey="Edson, Clark M" sort="Edson, Clark M" uniqKey="Edson C" first="Clark M." last="Edson">Clark M. Edson</name><name sortKey="Harn, Donald A" sort="Harn, Donald A" uniqKey="Harn D" first="Donald A." last="Harn">Donald A. Harn</name><name sortKey="Ianelli, Christopher J" sort="Ianelli, Christopher J" uniqKey="Ianelli C" first="Christopher J." last="Ianelli">Christopher J. Ianelli</name><name sortKey="Stadecker, Miguel J" sort="Stadecker, Miguel J" uniqKey="Stadecker M" first="Miguel J." last="Stadecker">Miguel J. 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